Status of foetal membranes is of foremost importance for the obstetrician ,mother and foetus .Once in the blue moon there are cases in which history is strongly in favor ruptured membranes ,but physical findings vibrate in other tunes enabling a doubt regarding status of foetal membranes .
To date a number of tests have been devised viz., Litmus pads test , Presence of Lanugos hairs , fat globules , Amniotic fluid crystallization test and Verni test.
A low cost simple ,reliable and risk free technique e is being presented based on the color changes on heating the material collected from endocervical canal.
The present study was carried out .The cases selected were having no history of ruptured membranes , physical findings in favor of presence of membranes ,cervical dilatation was at least 2 cms. and there was no contraindication for artificial rupture of membranes.
After confirming the presence of membranes by physical findings ,under full aseptic precautions the endocervical material was collected and was spread on the glass slide. Foetal membranes were ruptured and again the endocervical material was collected and spread over the slides. The contra lateral surfaces of both slides were heated for a minute by spirit lamp and color change of the material was noted.
In majority of cases in first slide color of endocervical material , changed to brown color on heating. On heating the second slide no color could be obtained , it turned to white., suggesting that in cases with intact foetal membranes on heating endocervical material , the color of material changed to brown., while in cases with ruptured membranes ( artificially ) the color changed to white.
The brown color that appears on heating endocervical materials from patients having intact foetal membranes is because of carbonization of proteins present in mucus, on the contrary , when membranes were ruptured the mucus get mixed with the amniotic fluid and on heating such a material the water of amniotic fluid evaporates and electrolytes present in amniotic fluid gives white color.
It is very well evident that this technique is so simple and without any risk that it can be used routinely on doubtful cases to assess the integrity of foetal membranes.
Published in: J.of obst. & Gynae. Of India : 37,59;1987
To date a number of tests have been devised viz., Litmus pads test , Presence of Lanugos hairs , fat globules , Amniotic fluid crystallization test and Verni test.
A low cost simple ,reliable and risk free technique e is being presented based on the color changes on heating the material collected from endocervical canal.
The present study was carried out .The cases selected were having no history of ruptured membranes , physical findings in favor of presence of membranes ,cervical dilatation was at least 2 cms. and there was no contraindication for artificial rupture of membranes.
After confirming the presence of membranes by physical findings ,under full aseptic precautions the endocervical material was collected and was spread on the glass slide. Foetal membranes were ruptured and again the endocervical material was collected and spread over the slides. The contra lateral surfaces of both slides were heated for a minute by spirit lamp and color change of the material was noted.
In majority of cases in first slide color of endocervical material , changed to brown color on heating. On heating the second slide no color could be obtained , it turned to white., suggesting that in cases with intact foetal membranes on heating endocervical material , the color of material changed to brown., while in cases with ruptured membranes ( artificially ) the color changed to white.The brown color that appears on heating endocervical materials from patients having intact foetal membranes is because of carbonization of proteins present in mucus, on the contrary , when membranes were ruptured the mucus get mixed with the amniotic fluid and on heating such a material the water of amniotic fluid evaporates and electrolytes present in amniotic fluid gives white color.
Published in: J.of obst. & Gynae. Of India : 37,59;1987
lot of thanx dr. saheb blog par unik chijen mil rahin hai sir
ReplyDeleteDear Dr. Raghav, I was thrilled to see this blog from my faculty. From bottom of my heart I congratulate you. The content are new one and that to combined with photos giving a nice information.
ReplyDeleteI salute you , My dear.
Thanks.
Dr. Mukesh Raghav Ji , I am very sorry to submit that , I had a child., by caeserean section. Concerned doctor told me that the membranes were puptured , hence we have decided for Caeseraen., otherwise your baby will die in utero.
ReplyDeleteWhy Drs. are making fool , as I did not had ruptured membranes. This article is for the benefit of your branch.
Regards.
Dr. Raghav,M. , I am surprised to read this article with beautiful photos, full of knowledge. Really you are a tag for the faculty.
ReplyDeleteSir, You are great !! Always try to favor resident doctors . Many a times , not daily in labor room posting , we are facing this problem. Now I salute a teacher like you Dr. Mukesh Raghav.
ReplyDelete